Additional sequence complexity is introduced by the addition or removal of nucleotides at the junctions of these segments. During the development of B and T cells, the variable antigen receptor gene segments are rearranged through targeted DNA recombination events. This protection is mediated by B and T lymphocytes and their receptors that bind pathogen derived antigens as well as major histocompatibility complex (MHC) bound peptides. The adaptive immune system shields the human body from a large variety of potential pathogens. The raw sequencing data have been uploaded to Zenodo (10.5281/zenodo.27483).įunding: This work was supported by Austrian Science Funds P25726 ( ) and European Regional Development Funds DAABAA_00720 ( ).Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: The authors confirm that all data underlying the findings are fully available without restriction. Received: Accepted: OctoPublished: November 23, 2015Ĭopyright: © 2015 Weinberger et al. PLoS ONE 10(11):Įditor: Grant Lythe, University of Leeds, UNITED KINGDOM (2015) Immune Repertoire Profiling Reveals that Clonally Expanded B and T Cells Infiltrating Diseased Human Kidneys Can Also Be Tracked in Blood. Our data suggest that expanded clonotypes in the tissues might be traceable in blood samples in the course of treatment or the natural history of the disease.Ĭitation: Weinberger J, Jimenez-Heredia R, Schaller S, Suessner S, Sunzenauer J, Reindl-Schwaighofer R, et al. Immune repertoire analysis of tissue infiltrating B and T cells adds new approaches to the assessment of adaptive immune response in kidney diseases. In addition, complementarity determining region 3 sequences of the immunoglobulin heavy chains are on average more diverse than T cell receptor beta chains. ![]() Summarizing the data of all analyzed patients, 68% of highly expanded T cell clonotypes and 30% of the highly expanded B cell clonotypes that have infiltrated the kidney can be found amongst the five most abundant clonotypes in blood. ![]() While generally an individual’s renal receptor repertoire is different from the repertoire present in blood, 94% (30/32) of the lymphocytes with clonal expansion in kidney can also be traced in blood however, not all of these clonotypes are equally abundant. We used Biomed-2 primer panels and ImmunExplorer software to sequence, analyze and compare complementarity determining regions and V-(D)-J elements. In this study we compared Immunoglobulin and T cell receptor repertoires of lymphocytes found in kidney and blood samples of 10 patients with various renal diseases based on next-generation sequencing data. Recent advances in high-throughput sequencing allow for the competitive analysis of the human B and T cell immune repertoire.
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